Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Dearen T[original query] |
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Common occurrence of a unique Cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the Chitwan National Park, Nepal
Feng Y , Raj Karna S , Dearen TK , Singh DK , Adhikari LN , Shrestha A , Xiao L . Vet Parasitol 2012 185 309-14 There are very few studies on the diversity and public health significance of Cryptosporidium species in zebu cattle and water buffaloes in developing countries. In this study, PCR-restriction fragment length polymorphism and DNA sequence analyses of the small-subunit (SSU) rRNA gene were used to genotype Cryptosporidium specimens from 12 zebu cattle calves, 16 water buffalo calves, and four swamp deer (Cervus duvaucelii) collected from the buffer zone of the Chitwan National Park, Nepal. All Cryptosporidium specimens from cattle and buffaloes belonged to Cryptosporidium ryanae, whereas those from deer belonged to Cryptosporidium ubiquitum. Comparison of the SSU rRNA gene sequences obtained with those from earlier studies has identified a nucleotide substitution unique to all C. ryanae isolates from Nepal, in addition to some sequence heterogeneity among different copies of the gene. The finding of the dominance of a unique C. ryanae variant in both zebu cattle and water buffaloes in Nepal indicates that there is unique cryptosporidiosis transmission in bovine animals in the study area, and cross-species transmission of some Cryptosporidium spp. can occur between related animal species sharing the same habitats. |
Prevalence, genetic characteristics, and zoonotic potential of Cryptosporidium species causing infections in farm rabbits in China
Shi K , Jian F , Lv C , Ning C , Zhang L , Ren X , Dearen TK , Li N , Qi M , Xiao L . J Clin Microbiol 2010 48 (9) 3263-6 To assess the prevalence and public health significance of rabbit cryptosporidiosis, a total of 1,081 fecal specimens were collected between October 2007 and April 2008 from rabbits on eight farms in five different areas in Henan Province, China, and were examined by microscopy after Sheather's sucrose flotation and modified acid-fast staining. The average infection rate of Cryptosporidium was 3.4% (37/1,081 samples). There was a significant association between the prevalence of Cryptosporidium and the age of animals (chi(2) = 57.13; P < 0.01); the prevalence of cryptosporidiosis in 1- to 3-month-old rabbits was the highest (10.9%). The Cryptosporidium species in microscopy-positive specimens were genotyped by sequence analyses of the 18S rRNA, 70-kDa heat shock protein (HSP70), oocyst wall protein (COWP), and actin genes and were subtyped by sequence analysis of the 60-kDa glycoprotein (gp60) gene. Only the Cryptosporidium rabbit genotype was identified, with 100% sequence identity to published sequences of the 18S rRNA, HSP70, COWP, and actin genes, and the strains belonged to three gp60 subtypes (VbA36, VbA35, and VbA29). In view of the recent finding of the Cryptosporidium rabbit genotype in human outbreak and sporadic cases, the role of rabbits in the transmission of human cryptosporidiosis should be reassessed. |
Subtype analysis of Cryptosporidium specimens from sporadic cases in Colorado, Idaho, New Mexico, and Iowa in 2007: widespread occurrence of one Cryptosporidium hominis subtype and case history of an infection with the Cryptosporidium horse genotype
Xiao L , Hlavsa MC , Yoder J , Ewers C , Dearen T , Yang W , Nett R , Harris S , Brend SM , Harris M , Onischuk L , Valderrama AL , Cosgrove S , Xavier K , Hall N , Romero S , Young S , Johnston SP , Arrowood M , Roy S , Beach MJ . J Clin Microbiol 2009 47 (9) 3017-20 Subtyping was conducted in late 2007 on 57 Cryptosporidium specimens from sporadic cases in Colorado, Idaho, New Mexico, and Iowa. One previously rare Cryptosporidium hominis subtype was identified in 40 cases (70%) from all four states, and the Cryptosporidium horse genotype was identified in a pet shop employee with severe clinical symptoms. |
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